Description
Principle of the assay: MAP2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MAP2. Standards or samples are then added to the microtiter plate wells and MAP2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MAP2 present in the sample, a standardized prepaHumanion of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for MAP2 are added to each well to "sandwich" the MAP2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substHumane solutions are added to each well. The enzyme (HRP) and substHumane are allowed to react over a short incubation period. Only those wells that contain MAP2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substHumane reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentHumanion of standards. The MAP2 concentHumanion in each sample is interpolated from this standard curve